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a Impedance magnitude and b phase recorded for 12 days of Caco-2 cell culture inside HuMiX. c Immunofluorescence staining of the epithelial Caco-2 barrier grown in the HuMiX for 12 days. Cell nuclei were stained with <t>DAPI</t> (blue) and the tight junction protein occludin was stained with Alexa Fluor 488 (green). The measurements were taken at electrode position 2 (~29 mm from the inlet of the device)
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a Impedance magnitude and b phase recorded for 12 days of Caco-2 cell culture inside HuMiX. c Immunofluorescence staining of the epithelial Caco-2 barrier grown in the HuMiX for 12 days. Cell nuclei were stained with <t>DAPI</t> (blue) and the tight junction protein occludin was stained with Alexa Fluor 488 (green). The measurements were taken at electrode position 2 (~29 mm from the inlet of the device)
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a Impedance magnitude and b phase recorded for 12 days of Caco-2 cell culture inside HuMiX. c Immunofluorescence staining of the epithelial Caco-2 barrier grown in the HuMiX for 12 days. Cell nuclei were stained with <t>DAPI</t> (blue) and the tight junction protein occludin was stained with Alexa Fluor 488 (green). The measurements were taken at electrode position 2 (~29 mm from the inlet of the device)
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a Impedance magnitude and b phase recorded for 12 days of Caco-2 cell culture inside HuMiX. c Immunofluorescence staining of the epithelial Caco-2 barrier grown in the HuMiX for 12 days. Cell nuclei were stained with DAPI (blue) and the tight junction protein occludin was stained with Alexa Fluor 488 (green). The measurements were taken at electrode position 2 (~29 mm from the inlet of the device)

Journal: Microsystems & Nanoengineering

Article Title: Integration of multiple flexible electrodes for real-time detection of barrier formation with spatial resolution in a gut-on-chip system

doi: 10.1038/s41378-023-00640-x

Figure Lengend Snippet: a Impedance magnitude and b phase recorded for 12 days of Caco-2 cell culture inside HuMiX. c Immunofluorescence staining of the epithelial Caco-2 barrier grown in the HuMiX for 12 days. Cell nuclei were stained with DAPI (blue) and the tight junction protein occludin was stained with Alexa Fluor 488 (green). The measurements were taken at electrode position 2 (~29 mm from the inlet of the device)

Article Snippet: The cells were labeled with occludin antibody diluted in 4% BSA for 3 h at room temperature, and nuclei were stained overnight with Fluoroshield TM with DAPI (F6057, Merck Milipore, Hoeilaart, Belgium).

Techniques: Cell Culture, Immunofluorescence, Staining